There are approximately 23 at-risk species in the Rouge which include:
- Peregrine Falcon
- Chimney Swift
- Common Nighthawk
- Bank swallow
- Hooded Warbler
- Red-headed Woodpecker
- Red-shouldered Hawk
- Red-side dace
- American Eel
- Blanding's Turtle
- Northern Map Turtle
- Milk snake
- Dense Blazing Star flower
- Butternut tree
- Bashful Bulrush
- Least bittern
- Monarch butterfly
