Eight years after Patricia Real was murdered, police are hoping new DNA evidence will help them crack the case.

Homicide detectives in Toronto held a news conference Wednesday morning to ask for more public tips on the death of the 46-year-old mother of two.

Real was gunned down on August 17, 2000 at her Etobicoke home soon after she arrived from her telemarketing job. Police say she was shot in the head twice at very close range.

She had just settled into her backyard with her boyfriend when a male suspect followed them onto the property. The suspect also struggled with the boyfriend but managed to get away.

Detectives have never made an arrest in the case, despite finding a bicycle they say was used by a suspect to flee the scene. The bike was found close to the crime scene and was tested for forensics.

At the news conference, Det. Sgt. Reg Pitts said results from testing recently completed on the bike have given investigators new clues.

"The initial investigation did not yield results but new testing reveals the DNA profiles of both a man and a woman," he said at the news conference. "The DNA belonged to Patti Real and someone else who is not on the DNA registry."

Pitts said he hopes new public information will help link the DNA evidence to a suspect.

Police say Real did not have a criminal past but that she had been associated with a criminal element in the last few years of her life.

She was dealing with a messy break-up from a man with ties to biker gang activity, police said.

The man, Ronald Harper, was seen at a casino at the time of the murder and was eliminated as the murder suspect. However, police have not ruled out the theory that the shooter was a hired gunman.

"It was very well executed," Pitts said at the news conference."The person came with a plan and made his escape."

In 2001, Harper committed suicide.

At the time of the murder, police released pictures of the bike and asked the public to contact them if it looked familiar to them.

The bicycle was described as a black mountain bike with chrome rims, a racing seat, knobby tires, front shock absorbers, straight handle bars and a missing hand grip on the left side. The make was identified as Mongoose and the model as Rockadile.

Police describe the male suspect in the case as tall and thin but they are unsure of his race. They say he was wearing a black puffy waist length jacket, black denim jeans and a black baseball cap.

Real left behind two sons from a previous relationship. Police say she was gainfully employed and was living by herself at the time of the murder.

With a report from CTV Toronto's Matet Nebres